Details

Autor(en) / Beteiligte

• Huiet, Layne

Titel

Molecular Analysis of the Neurospora qa-1 Regulatory Region Indicates That Two Interacting Genes Control qa Gene Expression

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 1984-02, Vol.81 (4), p.1174-1178

Ort / Verlag

United States: National Academy of Sciences of the United States of America

Erscheinungsjahr

1984

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The qa-1 regulatory region controls the expression of the three structural genes required for the early reactions in quinic acid in Neurospora crassa. Genetic analysis previously identified two types of noninducible qa-1 mutants, qa-1Sand qa-1F, which mapped in separate non-overlapping regions. These mutations were originally interpreted as defining separate domains of a single regulatory protein. This communication describes the further genetic and physical characterization of the qa-1 regulatory region. Using both Neurospora transformation and DNA· RNA hybridization, it has been shown that the qa-1 region consists of two distinct genes corresponding to the two original mutational types qa-1Sand qa-1F. The analysis of the mRNA species hybridizing to these regions indicates that the qa-1F gene encodes a 2.9-kilobase (kb) mRNA, while the qa-1S gene encodes related 4.1-kb and 3.4-kb mRNAs. The transcriptional regulation of one of these genes, qa-1S, was examined. Evidence is presented that the qa-1S gene is induced by quinic acid and is also subject to apparent autogenous regulation as well as to control by the qa-1F gene product. Based on these results and earlier genetic analysis, the hypothesis is proposed that one of the two qa regulatory genes encodes a repressor protein (qa-1S), and the other encodes an activator protein (qa-1F), both of which control qa gene expression.