Details

Autor(en) / Beteiligte

• Croze, F
• Franchimont, P

Titel

An in-vitro model for the study of inhibin production by rat ovarian cells

Ist Teil von

• Journal of reproduction & fertility, 1984-09, Vol.72 (1), p.249-260

Ort / Verlag

England

Erscheinungsjahr

1984

Quelle

MEDLINE

Beschreibungen/Notizen

• A culture system for ovarian cells from immature rats injected with PMSG was developed to study the production of inhibin. The results showed that 2 X 10(6) cells per culture dish (60 X 15 mm) were necessary for sustained production of inhibin and that serum was an obligatory component in the medium. Serum from bovine fetuses was better than serum from newborn calves for inhibin production. The constituents responsible for the maintenance of inhibin production by fetal bovine serum could be adsorbed with 1% charcoal and 0.1% dextran.