Details

Autor(en) / Beteiligte

• Krarup, Torben
• Loft, Hans

Titel

PRESENCE OF DMBA-3H IN THE MOUSE OVARY AND ITS RELATION TO OVARIAN TUMOUR INDUCTION

Ist Teil von

• Acta Pathologica Microbiologica Scandinavica Section A Pathology, 1971-07, Vol.79A (2), p.139-149

Ort / Verlag

Oxford, UK: Blackwell Publishing Ltd

Erscheinungsjahr

1971

Quelle

MEDLINE

Beschreibungen/Notizen

• Ovarian tumours in mice can be induced by 9:10‐dimethyl‐l: 2‐benzanthracene (DMBA). DMBA's primary action in the mouse ovary is the destruction of small oocytes. Whether the parent DMBA or some break‐down product is the effective carcinogen and whether prolonged retention of the carcinogenic principle in the target organ is a prerequisite for a neoplasma to develop are unknown. These questions were studied in experiments with tritiated DMBA, comparing the primary cytological effect of DMBA with the presence of radioactivity in tissue extracts. Radioactive compounds extracted from tissues after treatment with DMBA‐3H were analysed by two‐dimensional chromatography. The results suggest that DMBA itself is the effective initiator in ovary carcinogenesis in mice rather than some in vivo conversion of the chemical. The different rates at which small oocytes are destroyed after oral and intraperitoneal administration of the carcinogen can be directly correlated with different levels of carcinogen obtained in the tissues after the two routes of administration and to their different excretion rates. It is concluded, that the carcinogenic effect of DMBA on mouse ovaries is related to the immediate destruction of small oocytes, whereas the prolonged retention of DMBA after intraperitoneal injection is of no significance for the neoplastic development.