Details

Autor(en) / Beteiligte

• Higgs, Victoria E
• Toro-Tapia, Gabriela
• Burbidge, Holly B
• Das, Raman M

Titel

In Ovo Electroporation of Embryonic Chicken Spinal Cord in Combination with Ex Vivo Slice Culture for Live Imaging of Vertebrate Neuronal Differentiation

Ist Teil von

• Methods in molecular biology (Clifton, N.J.), 2024, Vol.2831, p.97

Ort / Verlag

United States

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the cell behavior underlying neuronal differentiation in a physiologically relevant context, differentiating neurons must be studied in their native tissue environment. Here, we describe an accessible protocol for fluorescent live imaging of differentiating neurons within ex vivo embryonic chicken spinal cord slice cultures, which facilitates long-term observation of individual cells within developing tissue.