Details

Autor(en) / Beteiligte

• Pérez-Isidoro, Rosendo
• Valdez-Lara, Alejandra Gabriela
• Díaz-Salazar, Alma Jessica
• Hoeppener, Stephanie
• Guerrero-Sánchez, Carlos
• Quintana-Owen, Patricia
• Ruiz-Suárez, Jesus Carlos
• Schubert, Ulrich S
• Ayora-Talavera, Guadalupe
• De Jesús-Téllez, Marco Antonio
• Saldívar-Guerra, Enrique

Titel

Biophysical investigation of liposome systems decorated with bioconjugated copolymers in the presence of amantadine

Ist Teil von

• Journal of materials chemistry. B, Materials for biology and medicine, 2024-06, Vol.12 (24), p.5823-5837

Ort / Verlag

England: Royal Society of Chemistry

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• Liposome-based technologies derived from lipids and polymers ( e.g. , PEGylated liposomes) have been recognized because of their applications in nanomedicine. However, since such systems represent myriad challenges and may promote immune responses, investigation of new biomaterials is mandatory. Here, we report on a biophysical investigation of liposomes decorated with bioconjugated copolymers in the presence (or absence) of amantadine (an antiviral medication). First, copolymers of poly( N , N -dimethylacrylamide- co -fluoresceinacrylate- co -acrylic acid- N -succinimide ester)- block -poly( N -isopropylacrylamide) (PDMA- b -PNIPAM) containing a fluorescence label were biofunctionalized with short peptides that resemble the sequence of the loops 220 and 130 of the binding receptor of the hemagglutinin (HA) protein of the influenza A virus. Then, the bioconjugated copolymers were self-assembled along with liposomes composed of 1,2 dimyristoyl- sn-glycero -3-phosphocholine, sphingomyelin, and cholesterol (MSC). These biohybrid systems, with and without amantadine, were systematically characterized using differential scanning calorimetry (DSC), dynamic light scattering (DLS), and cryogenic transmission electron microscopy (cryoTEM). Finally, the systems were tested in an in vitro study to evaluate cytotoxicity and direct immunofluorescence in Madin Darbin Canine Kidney (MDCK) cells. The biohybrid systems displayed long-term stability, thermo-responsiveness, hydrophilic-hydrophobic features, and fluorescence properties and were presumable endowed with cell targeting properties intrinsically integrated into the amino acid sequences of the utilized peptides, which indeed turn them into promising nanodevices for biomedical applications. Self-assembled nanostructures derived from lipids and polymers are investigated as potential drug delivery vehicles in nanomedicine.