Details

Autor(en) / Beteiligte

• Chen, Ying
• Qiu, Mingyue
• Hu, Ruoqun
• Cao, Jiayu
• Liang, Wanfeng
• Yan, Shujun

Titel

Uncovering the molecular mechanisms behind steroidal saponin accumulation in Liriope muscari (Decne.) Baily through transcriptome sequencing and bioinformatics analysis

Ist Teil von

• Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2024-04, Vol.40 (4), p.1120

Ort / Verlag

China

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• The leaves and roots of (Decne.) Baily were subjected to high-throughput Illumina transcriptome sequencing. Bioinformatics analysis was used to investigate the enzyme genes and key transcription factors involved in regulating the accumulation of steroidal saponins, which are the main active ingredient in . . These analyses aimed to reveal the molecular mechanism behind steroidal saponin accumulation. The sequencing results of . revealed 31 enzymes, including , , and , that are involved in the synthesis of steroidal saponins. Among these enzymes, 16 were in the synthesis of terpenoid skeleton, 3 were involved in the synthesis of sesquiterpene and triterpene, and 12 were involved in the synthesis of steroidal compound. Differential gene expression identified 15 metabolic enzymes coded by 34 differentially expressed genes (DEGs) in the leaves and roots, which were associated with steroidal saponin synthesis. Further analysis using gene co-expression patterns showed that 14 metabolic enzymes coded by 31 DEGs were