Details

Autor(en) / Beteiligte

• Le, T H U T H U Y
• Huang, Y
• Xie, J P

Titel

Application and optimization of CRISPRi to the biology of Mycobacterium tuberculosis

Ist Teil von

• Zhonghua jiehe he huxi zazhi, 2024-04, Vol.47 (4), p.376

Ort / Verlag

China

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• Tuberculosis, caused by infection with (MTB), remains a global public health challenge. Multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) strains make tuberculosis more difficult to control. New tools to study the biology of MTB can identify novel targets for drug discovery. Recently, the Clustered Regularly Interspaced Short Palindromic Repeats interference (CRISPRi) combined with next-generation sequencing has provided many novel insights into the physiology and genetics of MTB. This review summarizes the application and optimization of CRISPRi in MTB biology.