Details

Autor(en) / Beteiligte

• Chen, H
• Li, Z
• Wang, M
• Lu, L
• Tang, Q
• Luo, L

Titel

High expression of UBE2S promotes progression of hepatocellular carcinoma by increasing cancer cell stemness

Ist Teil von

• Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2024-03, Vol.44 (3), p.455

Ort / Verlag

China

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the expression of the ubiquitination enzyme UBE2S in different cell types in hepatocellular carcinoma (HCC) microenvironment and its impact on proliferation and stemness of HCC cells. TCGA and CPTAC database were used to analyze the transcriptional and promoter methylation levels and protein expressions of UBE2S in HCC. Specific expression patterns of UBE2S, intercellular communication and key transcription factors in different cell types were analyzed based on single-cell sequencing data from TISCH website. We further examined UBE2S expressions in clinical samples of HCC tissues, HCC cells and T cells using immunohistochemistry and immunofluorescence staining. We also tested the effects of UBE2S knockdown on stemness of HCC-LM3 and HepG2 cells using clone formation experiments and sphere formation assay. Analysis based on TCGA database suggested significant overexpression of UBE2S in both paired and non-paired tumor tissues ( < 0.001), and its transcriptional level increased with tumor grades.