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Effect of TRIM59 Expression Interference on Daunorubicin Chemosensitivity of Chronic Myeloid Leukemia K562 Cells and Its Mechanism
Ist Teil von
Zhongguo shi yan xue ye xue za zhi, 2024-02, Vol.32 (1), p.45
Ort / Verlag
China
Erscheinungsjahr
2024
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
To investigate the effect of tripipartite motif 59 (TRIM59) expression interference on the chemosensitivity of daunorubicin (DNR) in chronic myeloid leukemia (CML) K562 cells and the related molecular mechanism.
The expressions of
mRNA in bone marrow tissues of patients with CML and K562 cells were detected by RT-qPCR. Liposome-based transfection technology was used to transfect TRIM59-specific siRNA (si-TRIM59) into K562 cells which then were treated with DNR. The proliferation and apoptosis of cells were detected by CCK-8 assay and flow cytometry, respectively, and the expressions of apoptosis-related protein and Wnt/β-catenin signaling pathway-related protein were detected by Western blot.
Compared with the bone marrow tissue of CML patients at the time of initial treatment, the expression of
mRNA in bone marrow tissue of CML patients at the time of chemotherapy resistance was significantly increased (
<0.05). Compared with control group, the cell proliferation inhibition rate and apoptosis rate in si-
gro