Details

Autor(en) / Beteiligte

• Antia, Imeobong U
• Hills, Frank A
• Shah, Ajit J

Titel

Disaccharide compositional analysis of chondroitin sulphate using WAX HILIC-MS with pre-column procainamide labelling; application to the placenta in pre-eclampsia

Ist Teil von

• Analytical methods, 2024-01, Vol.16 (4), p.566-575

Ort / Verlag

England: Royal Society of Chemistry

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• Chondroitin sulphate (CS) and dermatan sulphate are negatively charged linear heteropolysaccharides. These glycosaminoglycans (GAG) are involved in cellular signalling via binding to growth factors. CS is expressed in a range of tissue and biological fluids and is highly expressed in the placenta. There is evidence that decorin; a CS proteoglycan is significantly decreased in pre-eclampsia and fetal growth restriction. It is considered that GAG chain composition may influence cellular processes that are altered in pre-eclampsia. The goal of the present study was to develop an LC-MS method with precolumn procainamide labelling for the disaccharide compositional analysis of CS. The method was used to investigate whether the disaccharide composition of placenta-extracted CS is altered in pre-eclampsia. The study revealed differential disaccharide compositions of placental chondroitin sulphate between pre-eclampsia and other pregnancy conditions. This suggests that the method may have diagnostic potential for pregnancy disorders. Furthermore, the findings suggest that CS sulphation might play a significant role in maternal labour. The disaccharide composition of placental chondroitin sulphate was analysed following pre-column labelling. This revealed changes in chondroitin sulphate in various pregnancy conditions and showed increased sulphation is related to maternal labour.