Details

Autor(en) / Beteiligte

• Weng, Yicheng
• Chen, Wendong
• Kong, Qian
• Wang, Ruixiang
• Zeng, Ruxin
• He, An
• Liu, Yanjun
• Mao, Yiheng
• Qin, Yunqiu
• Ngai, William Shu Ching
• Zhang, Heng
• Ke, Mi
• Wang, Jie
• Tian, Ruijun
• Chen, Peng R

Titel

DeKinomics pulse-chases kinase functions in living cells

Ist Teil von

• Nature chemical biology, 2024-05, Vol.20 (5), p.615-623

Ort / Verlag

United States

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• Cellular context is crucial for understanding the complex and dynamic kinase functions in health and disease. Systematic dissection of kinase-mediated cellular processes requires rapid and precise stimulation ('pulse') of a kinase of interest, as well as global and in-depth characterization ('chase') of the perturbed proteome under living conditions. Here we developed an optogenetic 'pulse-chase' strategy, termed decaging kinase coupled proteomics (DeKinomics), for proteome-wide profiling of kinase-driven phosphorylation at second-timescale in living cells. We took advantage of the 'gain-of-function' feature of DeKinomics to identify direct kinase substrates and further portrayed the global phosphorylation of understudied receptor tyrosine kinases under native cellular settings. DeKinomics offered a general activation-based strategy to study kinase functions with high specificity and temporal resolution under living conditions.