Details

Autor(en) / Beteiligte

• Djennane, Samia
• Gersch, Sophie
• Le-Bohec, Françoise
• Piron, Marie-Christine
• Baltenweck, Raymonde
• Lemaire, Olivier
• Merdinoglu, Didier
• Hugueney, Philippe
• Nogué, Fabien
• Mestre, Pere
• Höfte, Monica

Titel

CRISPR/Cas9 editing of Downy mildew resistant 6 ( DMR6-1 ) in grapevine leads to reduced susceptibility to Plasmopara viticola

Ist Teil von

• Journal of experimental botany, 2024-03, Vol.75 (7), p.2100-2112

Erscheinungsjahr

2024

Beschreibungen/Notizen

• Abstract Downy mildew of grapevine (Vitis vinifera), caused by the oomycete Plasmopara viticola, is an important disease that is present in cultivation areas worldwide, and using resistant varieties provides an environmentally friendly alternative to fungicides. DOWNY MILDEW RESISTANT 6 (DMR6) from Arabidopsis is a negative regulator of plant immunity and its loss of function confers resistance to downy mildew. In grapevine, DMR6 is present in two copies, named VvDMR6-1 and VvDMR6-2. Here, we describe the editing of VvDMR6-1 in embryogenic calli using CRISPR/Cas9 and the regeneration of the edited plants. All edited plants were found to be biallelic and chimeric, and whilst they all showed reduced growth compared with non-transformed control plants, they also had reduced susceptibility to P. viticola. Comparison between mock-inoculated genotypes showed that all edited lines presented higher levels of salicylic acid than controls, and lines subjected to transformation presented higher levels of cis-resveratrol than controls. Our results identify VvDMR6-1 as a promising target for breeding grapevine cultivars with improved resistance to downy mildew.