Details

Autor(en) / Beteiligte

• Ford, Emily S
• Mayer-Blackwell, Koshlan
• Jing, Lichen
• Laing, Kerry J
• Sholukh, Anton M
• St Germain, Russell
• Bossard, Emily L
• Xie, Hong
• Pulliam, Thomas H
• Jani, Saumya
• Selke, Stacy
• Burrow, Carlissa J
• McClurkan, Christopher L
• Wald, Anna
• Greninger, Alexander L
• Holbrook, Michael R
• Eaton, Brett
• Eudy, Elizabeth
• Murphy, Michael
• Postnikova, Elena
• Robins, Harlan S
• Elyanow, Rebecca
• Gittelman, Rachel M
• Ecsedi, Matyas
• Wilcox, Elise
• Chapuis, Aude G
• Fiore-Gartland, Andrew
• Koelle, David M

Titel

Repeated mRNA vaccination sequentially boosts SARS-CoV-2-specific CD8 + T cells in persons with previous COVID-19

Ist Teil von

• Nature immunology, 2024-01, Vol.25 (1), p.166-177

Ort / Verlag

United States

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) hybrid immunity is more protective than vaccination or previous infection alone. To investigate the kinetics of spike-reactive T (T ) cells from SARS-CoV-2 infection through messenger RNA vaccination in persons with hybrid immunity, we identified the T cell receptor (TCR) sequences of thousands of index T cells and tracked their frequency in bulk TCRβ repertoires sampled longitudinally from the peripheral blood of persons who had recovered from coronavirus disease 2019 (COVID-19). Vaccinations led to large expansions in memory T cell clonotypes, most of which were CD8 T cells, while also eliciting diverse T cell clonotypes not observed before vaccination. TCR sequence similarity clustering identified public CD8 and CD4 TCR motifs associated with spike (S) specificity. Synthesis of longitudinal bulk ex vivo single-chain TCRβ repertoires and paired-chain TCRɑβ sequences from droplet sequencing of T cells provides a roadmap for the rapid assessment of T cell responses to vaccines and emerging pathogens.