Details

Autor(en) / Beteiligte

• Turiello, R
• Nouwairi, R. L
• Keller, J
• Cunha, L. L
• Dignan, L. M
• Landers, J. P

Titel

A rotationally-driven dynamic solid phase sodium bisulfite conversion disc for forensic epigenetic sample preparation

Ist Teil von

• Lab on a chip, 2023-12, Vol.24 (1), p.97-112

Ort / Verlag

England: Royal Society of Chemistry

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• The approaches to forensic human identification (HID) are largely comparative in nature, relying upon the comparison of short tandem repeat profiles to known reference materials and/or database profiles. However, many profiles are generated from evidence materials that either do not have a reference material for comparison or do not produce a database hit. As an alternative to individualizing analysis for HID, researchers of forensic DNA have demonstrated that the human epigenome can provide a wealth of information. However, epigenetic analysis requires sodium b&cmb.b.line;is&cmb.b.line;ulfite c&cmb.b.line;onversion (BSC), a sample preparation method that is time-consuming, labor-intensive, prone to contamination, and characterized by DNA loss and fragmentation. To provide an alternative method for BSC that is more amenable to integration with the forensic DNA workflow, we describe a rotationally-driven, microfluidic method for dynamic solid phase-BSC (dSP-BSC) that streamlines the sample preparation process in an automated format, capable of preparing up to four samples in parallel. The method permitted decreased incubation intervals by ∼36% and was assessed for relative DNA recovery and conversion efficiency and compared to gold-standard and enzymatic approaches. An alternative method for epigenetic sample preparation by a rotationlly-driven, microfluidic sodium bisulfite conversion system for up to four samples in parallel.