Details

Autor(en) / Beteiligte

• Nikopoulou, Chrysa
• Kleinenkuhnen, Niklas
• Parekh, Swati
• Sandoval, Tonantzi
• Ziegenhain, Christoph
• Schneider, Farina
• Giavalisco, Patrick
• Donahue, Kat-Folz
• Vesting, Anna Juliane
• Kirchner, Marcel
• Bozukova, Mihaela
• Vossen, Christian
• Altmüller, Janine
• Wunderlich, Thomas
• Sandberg, Rickard
• Kondylis, Vangelis
• Tresch, Achim
• Tessarz, Peter

Titel

Spatial and single-cell profiling of the metabolome, transcriptome and epigenome of the aging mouse liver

Ist Teil von

• Nature aging, 2023-11, Vol.3 (11), p.1430

Ort / Verlag

United States

Erscheinungsjahr

2023

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Tissues within an organism and even cell types within a tissue can age with different velocities. However, it is unclear whether cells of one type experience different aging trajectories within a tissue depending on their spatial location. Here, we used spatial transcriptomics in combination with single-cell ATAC-seq and RNA-seq, lipidomics and functional assays to address how cells in the male murine liver are affected by age-related changes in the microenvironment. Integration of the datasets revealed zonation-specific and age-related changes in metabolic states, the epigenome and transcriptome. The epigenome changed in a zonation-dependent manner and functionally, periportal hepatocytes were characterized by decreased mitochondrial fitness, whereas pericentral hepatocytes accumulated large lipid droplets. Together, we provide evidence that changing microenvironments within a tissue exert strong influences on their resident cells that can shape epigenetic, metabolic and phenotypic outputs.