Details

Autor(en) / Beteiligte

• Zhou, Jessica L
• de Guglielmo, Giordano
• Ho, Aaron J
• Kallupi, Marsida
• Pokhrel, Narayan
• Li, Hai-Ri
• Chitre, Apurva S
• Munro, Daniel
• Mohammadi, Pejman
• Carrette, Lieselot L G
• George, Olivier
• Palmer, Abraham A
• McVicker, Graham
• Telese, Francesca

Titel

Single-nucleus genomics in outbred rats with divergent cocaine addiction-like behaviors reveals changes in amygdala GABAergic inhibition

Ist Teil von

• Nature neuroscience, 2023-11, Vol.26 (11), p.1868

Ort / Verlag

United States

Erscheinungsjahr

2023

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The amygdala processes positive and negative valence and contributes to addiction, but the cell-type-specific gene regulatory programs involved are unknown. We generated an atlas of single-nucleus gene expression and chromatin accessibility in the amygdala of outbred rats with high and low cocaine addiction-like behaviors following prolonged abstinence. Differentially expressed genes between the high and low groups were enriched for energy metabolism across cell types. Rats with high addiction index (AI) showed increased relapse-like behaviors and GABAergic transmission in the amygdala. Both phenotypes were reversed by pharmacological inhibition of the glyoxalase 1 enzyme, which metabolizes methylglyoxal-a GABA receptor agonist produced by glycolysis. Differences in chromatin accessibility between high and low AI rats implicated pioneer transcription factors in the basic helix-loop-helix, FOX, SOX and activator protein 1 families. We observed opposite regulation of chromatin accessibility across many cell types. Most notably, excitatory neurons had greater accessibility in high AI rats and inhibitory neurons had greater accessibility in low AI rats.