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Construction of a Mouse Model for Myeloproliferative Neoplasms and an Evaluation System
Ist Teil von
Zhongguo shi yan xue ye xue za zhi, 2023-08, Vol.31 (4), p.1113
Ort / Verlag
China
Erscheinungsjahr
2023
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
To construct a myeloproliferative neoplasms (MPN) transplanted mouse model with
or
gene mutation, and establish a systematic evaluation system to verify the success of model construction.
The bone marrow c-kit
cells of the mice were obtained by the following steps: The mice were killed by cervical dislocation, the femur, tibia and ilium were separated, and the bone marrow cells were collected. The c-kit
cells were sorted after incubation with CD117 magnetic beads. The method of constructing mouse primary mutant cells is as follows: A gene mutation vector with a GFP tag was constructed by the retroviral system, and the retroviral vector was packaged into the Platinum-E cells to obtain the virus supernatant, and then used it to infect the c-kit
cells of mice. The MPN mouse model was constructed as follows: the mouse primary c-kit
cells containing the mutant genes were collected after infection, and then transplanted them via the tail vein into the female recipient mice of the same species which were irradiated