Details

Autor(en) / Beteiligte

• von Bibra, Constantin
• Shibamiya, Aya
• Bähr, Andrea
• Geertz, Birgit
• Köhne, Maria
• Stuedemann, Tim
• Starbatty, Jutta
• Horneffer-van der Sluis, Verena
• Klostermeier, Ulrich C
• Hornaschewitz, Nadja
• Li, Xinghai
• Wolf, Eckhard
• Klymiuk, Nikolai
• Krane, Markus
• Kupatt, Christian
• Hiebl, Bernhard
• Eschenhagen, Thomas
• Weinberger, Florian

Titel

Immature human engineered heart tissues engraft in a guinea pig chronic injury model

Ist Teil von

• Disease models & mechanisms, 2023-05, Vol.16 (5)

Ort / Verlag

England

Erscheinungsjahr

2023

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Engineered heart tissue (EHT) transplantation represents an innovative, regenerative approach for heart failure patients. Late preclinical trials are underway, and a first clinical trial started recently. Preceding studies revealed functional recovery after implantation of in vitro-matured EHT in the subacute stage, whereas transplantation in a chronic injury setting was less efficient. When transplanting matured EHTs, we noticed that cardiomyocytes undergo a dedifferentiation step before eventually forming structured grafts. Therefore, we wanted to evaluate whether immature EHT (EHTIm) patches can be used for transplantation. Chronic myocardial injury was induced in a guinea pig model. EHTIm (15×106 cells) were transplanted within hours after casting. Cryo-injury led to large transmural scars amounting to 26% of the left ventricle. Grafts remuscularized 9% of the scar area on average. Echocardiographic analysis showed some evidence of improvement of left-ventricular function after EHTIm transplantation. In a small translational proof-of-concept study, human scale EHTIm patches (4.5×108 cells) were epicardially implanted on healthy pig hearts (n=2). In summary, we provide evidence that transplantation of EHTIm patches, i.e. without precultivation, is feasible, with similar engraftment results to those obtained using matured EHT.