Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2023-04, Vol.43 (4), p.560
2023
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- Autor(en) / Beteiligte
- Titel
- Silencing RAB27a inhibits proliferation, invasion and adhesion of triple-negative breast cancer cells
- Ist Teil von
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2023-04, Vol.43 (4), p.560
- Ort / Verlag
- China
- Erscheinungsjahr
- 2023
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To investigate the effect of inhibition of RAB27 protein family, which plays a pivotal role in exosome secretion, on biological behaviors of triple-negative breast cancer cells. Quantitative real-time PCR and Western blotting were used to examine the expressions of RAB27 family and exosome secretion in 3 triple-negative breast cancer cell lines (MDA-MB-231, MDA-MB-468, and Hs578T) and a normal breast epithelial cell line (MCF10A). The effect of small interfering RNA (siRNA)-mediated silencing of RAB27a and RAB27b on exosome secretion in the 3 breast cancer cell lines was detected using Western blotting, and the changes in cell proliferation, invasion and adhesion were evaluated. Compared with normal breast epithelial cells, the 3 triple-negative breast cancer cell lines exhibited more active exosome secretion ( < 0.001) and showed significantly higher expressions of RAB27a and RAB27b at both the mRNA and protein levels ( < 0.01). Silencing of RAB27a in the breast cancer cells significantly down-regulated exos
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1673-4254DOI: 10.12122/j.issn.1673-4254.2023.04.08Titel-ID: cdi_pubmed_primary_37202191
- Format
- –
- Schlagworte
- Cell Line, Tumor, Cell Proliferation - genetics, Gene Expression Regulation, Neoplastic, Humans, rab GTP-Binding Proteins - genetics, rab GTP-Binding Proteins - metabolism, rab27 GTP-Binding Proteins - genetics, rab27 GTP-Binding Proteins - metabolism, RNA, Small Interfering - genetics, Triple Negative Breast Neoplasms