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Silencing RAB27a inhibits proliferation, invasion and adhesion of triple-negative breast cancer cells
Ist Teil von
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2023-04, Vol.43 (4), p.560
Ort / Verlag
China
Erscheinungsjahr
2023
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
To investigate the effect of inhibition of RAB27 protein family, which plays a pivotal role in exosome secretion, on biological behaviors of triple-negative breast cancer cells.
Quantitative real-time PCR and Western blotting were used to examine the expressions of RAB27 family and exosome secretion in 3 triple-negative breast cancer cell lines (MDA-MB-231, MDA-MB-468, and Hs578T) and a normal breast epithelial cell line (MCF10A). The effect of small interfering RNA (siRNA)-mediated silencing of RAB27a and RAB27b on exosome secretion in the 3 breast cancer cell lines was detected using Western blotting, and the changes in cell proliferation, invasion and adhesion were evaluated.
Compared with normal breast epithelial cells, the 3 triple-negative breast cancer cell lines exhibited more active exosome secretion (
< 0.001) and showed significantly higher expressions of RAB27a and RAB27b at both the mRNA and protein levels (
< 0.01). Silencing of RAB27a in the breast cancer cells significantly down-regulated exos