Details

Autor(en) / Beteiligte

• Samur, Mehmet Kemal
• Szalat, Raphael
• Munshi, Nikhil C.

Titel

Single-cell profiling in multiple myeloma: insights, problems, and promises

Ist Teil von

• Blood, 2023-07, Vol.142 (4), p.313-324

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• [Display omitted] In a short time, single-cell platforms have become the norm in many fields of research, including multiple myeloma (MM). In fact, the large amount of cellular heterogeneity in MM makes single-cell platforms particularly attractive because bulk assessments can miss valuable information about cellular subpopulations and cell-to-cell interactions. The decreasing cost and increasing accessibility of single-cell platform, combined with breakthroughs in obtaining multiomics data for the same cell and innovative computational programs for analyzing data, have allowed single-cell studies to make important insights into MM pathogenesis; yet, there is still much to be done. In this review, we will first focus on the types of single-cell profiling and the considerations for designing a single-cell profiling experiment. Then, we will discuss what have learned from single-cell profiling about myeloma clonal evolution, transcriptional reprogramming, and drug resistance, and about the MM microenvironment during precursor and advanced disease. Single-cell profiling technologies can assess the genome, transcriptome, and epigenome of individual cells at high resolution. When combined, they allow recognition of cell identity, state, and activity. Samur and colleagues review how these technical and bioinformatic advances have shed light on how myeloma can become drug-resistant and the nature of the bidirectional relationship between clonal evolution and the changing tumor microenvironment.