Details

Autor(en) / Beteiligte

• Vorobjev, Ivan A
• Kussanova, Aigul
• Barteneva, Natasha S

Titel

Development of Spectral Imaging Cytometry

Ist Teil von

• Methods in molecular biology (Clifton, N.J.), 2023, Vol.2635, p.3

Ort / Verlag

United States

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Spectral flow cytometry is a new technology that enables measurements of fluorescent spectra and light scattering properties in diverse cellular populations with high precision. Modern instruments allow simultaneous determination of up to 40+ fluorescent dyes with heavily overlapping emission spectra, discrimination of autofluorescent signals in the stained specimens, and detailed analysis of diverse autofluorescence of different cells-from mammalian to chlorophyll-containing cells like cyanobacteria. In this paper, we review the history, compare modern conventional and spectral flow cytometers, and discuss several applications of spectral flow cytometry.