Details

Autor(en) / Beteiligte

• Maassen, Hanno
• Venema, Leonie H
• Weiss, Marc G
• Huijink, Tobias M
• Hofker, H Sijbrand
• Keller, Anna K
• Mollnes, Tom E
• Eijken, Marco
• Pischke, Søren E
• Jespersen, Bente
• van Goor, Harry
• Leuvenink, Henri G D

Titel

H 2 S-Enriched Flush out Does Not Increase Donor Organ Quality in a Porcine Kidney Perfusion Model

Ist Teil von

• Antioxidants, 2023-03, Vol.12 (3)

Ort / Verlag

Switzerland

Erscheinungsjahr

2023

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Kidney extraction time has a detrimental effect on post-transplantation outcome. This study aims to improve the flush-out and potentially decrease ischemic injury by the addition of hydrogen sulphide (H S) to the flush medium. Porcine kidneys ( = 22) were extracted during organ recovery surgery. Pigs underwent brain death induction or a Sham operation, resulting in four groups: donation after brain death (DBD) control, DBD H S, non-DBD control, and non-DBD H S. Directly after the abdominal flush, kidneys were extracted and flushed with or without H S and stored for 13 h via static cold storage (SCS) +/- H S before reperfusion on normothermic machine perfusion. Pro-inflammatory cytokines IL-1b and IL-8 were significantly lower in H S treated DBD kidneys during NMP ( = 0.03). The non-DBD kidneys show superiority in renal function (creatinine clearance and FENa) compared to the DBD control group ( = 0.03 and = 0.004). No differences were seen in perfusion parameters, injury markers and histological appearance. We found an overall trend of better renal function in the non-DBD kidneys compared to the DBD kidneys. The addition of H S during the flush out and SCS resulted in a reduction in pro-inflammatory cytokines without affecting renal function or injury markers.