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Aequorivita vitellina sp. nov. and Aequorivita xiaoshiensis sp. nov., isolated from marine sediment
Ist Teil von
International journal of systematic and evolutionary microbiology, 2023-01, Vol.73 (3)
Ort / Verlag
England
Erscheinungsjahr
2023
Quelle
MEDLINE
Beschreibungen/Notizen
Two Gram-stain-negative, strictly aerobic, chemoheterotrophic, short-rod-shaped and non-motile strains, forming yellow colonies and designated F47161
and F64183
, were isolated from marine sediment of Xiaoshi Island, Wei Hai, PR China. Strain F47161
grew at 15-37 °C (optimally at 30 °C) and pH 6.0-9.0 (optimally at pH 7.5) and in the presence of 1-9 % (w/v) NaCl (optimally at 3 %). Strain F64183
grew at 10-37 °C (optimally at 30 °C) and pH 6.0-8.5 (optimally at pH 7.0) and in the presence of 1-8 % (w/v) NaCl (optimally at 3 %). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that F47161
and F64183
were related to members of the genus
. The strains shared 97.4 % 16S rRNA gene sequence similarity to each other. F47161
and F64183
shared highest 16S rRNA gene sequence similarity to
JCM 19789
, and the values were 97.5 % and 98.4 %, respectively. The predominant cellular fatty acids of both F64183
and F47161
were iso-C
and iso-C
3-OH, but the predominant fatty acids of F47161
also included anteiso-C
. The sole respiratory quinone of F47161
and F64183
was menaquinone 6 (MK-6), consistent with that observed for all related strains. Between F47161
and F64183
, the average nucleotide identity (ANI) and digital DNA-DNA hybridisation (dDDH) values were 75.8 % and 20.5 %, respectively, and between the novel isolates (F47161
and F64183
) and
JCM 19789
they were 76.0 % and 94.2 % and 20.6 % and 57.1 %, respectively. The genomic DNA G+C contents of F47161
and F64183
was 37.3 % and 34.5 %, respectively. The polar lipid profiles of F47161
and F64183
contained phosphatidylethanolamine, two aminolipids, one glycolipid, one phosphoglycolipid and two unidentified polar lipids. Differential phenotypic and genotypic characteristics of the two strains indicated that the two strains should be classified as representing two novel species of the genus
for which the names
sp. nov. and
sp. nov. are proposed. The type strains are F47161
(=MCCC 1H00509
=KCTC 92017
) and F64183
(=MCCC 1H00507
=KCTC 92016
), respectively.