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Porphyromonas gingivalis infection causes umbilical vein endothelial barrier dysfunction in vitro by down-regulating ZO-1, occludin and VE-cadherin expression
Ist Teil von
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2023-02, Vol.43 (2), p.287
Ort / Verlag
China
Erscheinungsjahr
2023
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
To explore the molecular mechanisms of
infection-induced umbilical vein endothelial barrier dysfunction
.
Human umbilical vein endothelial cells (HUVECs) were cultured
, and after the formation of the endothelial barrier, the cells were infected with
at a multiplicity of infection (MOI). The transepithelial electrical resistance (TEER) of the cell barrier was measured, and FITC-dextran trans-endothelial permeability assay and bacterial translocation assay were performed to assess the endothelial barrier function. The expression levels of cell junction proteins including ZO-1, occludin and VE-cadherin in the cells were examined by qRT-PCR and Western blotting.
In freshly seeded HUVECs, TEER increased until reaching the maximum on Day 5 (94 Ωcm
), suggesting the formation of the endothelial barrier.
infection caused an increase of the permeability of the endothelial barrier as early as 0.5 h after bacterial inoculation, and the barrier function further exacerbated with time, as shown by significantly lowered TE