Details

Autor(en) / Beteiligte

• Wong, Cherie CY
• Sun, Lu-Lu
• Liu, Meng-Jiao
• Stride, Eleanor
• Raymond, Jason L
• Han, Hai-Hao
• Kwan, James
• Sedgwick, Adam C

Titel

Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells

Ist Teil von

• Chemical communications (Cambridge, England), 2023-04, Vol.59 (29), p.4328-4331

Ort / Verlag

England: Royal Society of Chemistry

Erscheinungsjahr

2023

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• We report the synthesis of hydroxyl-radical (&z.rad;OH) responsive fluorescent probes that utilise the 3,5-dihydroxybenzyl (DHB) functionality. 4-Methylumbeliferone-DHB (Umb-DHB) and resorufin-DHB (Res-DHB) in the presence of &z.rad;OH radicals resulted in significant increases in their respective fluorescent emission intensities at 460 nm and 585 nm. The incubation of Res-DHB in HeLa cells followed by therapeutic ultrasound (1 MHz) resulted in a significant increase in fluorescence emission intensity thus permitting the ability to monitor ultrasound-induced &z.rad;OH production in live cells. Hydroxyl-radical (&z.rad;OH) responsive fluorescent probes (Umb-DHB and Res-DHB) were developed that utilise the 3,5-dihydroxybenzyl (DHB) functionality. Res-DHB responded to ultrasound-induced hydroxyl radical production in solution and in cells.