Details

Autor(en) / Beteiligte

• Wang, Yue
• Liu, Xiao-Qian
• Zhang, Yong-Xin
• Feng, Wei-Hong
• Yan, Li-Hua
• Wang, Zhi-Min

Titel

Quality evaluation methods of Poria based on yield and content of alkali extracted polysaccharide

Ist Teil von

• Zhongguo zhongyao zazhi, 2023-01, Vol.48 (2), p.443

Ort / Verlag

China

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• To improve the quality control methods of Poria and develop and utilize its resources fully, alkaline extraction was used in this study to determine the yield and content of alkali-soluble polysaccharides of Poria. The alkali-soluble extracts of Poria were obtained according to the optimum extraction conditions on the basis of single-factor test, and 30 batches of samples were determined. The structure and chemical composition of the alkali-soluble extracts was characterized by high-performance gel permeation chromatography(HPGPC), Fourier transform infrared spectrometry(FT-IR), nuclear magnetic resonance(NMR) spectroscopy and high-performance liquid chromatography(HPLC) with 1-phenyl-3-methyl-5-pyrazolone(PMP-HPLC). The results showed that the content of the alkali-soluble extracts was in the range of 46.98%-73.86%. The main component was β-(1→3)-glucan, and its molecular mass was about 1.093×10~5. Further, the content of alkali-soluble polysaccharides of Poria was measured by UV-Vis spectrophotometry and HP