Details

Autor(en) / Beteiligte

• Salehi, Hamideh
• Ramoji, Anuradha
• Mougari, Said
• Merida, Peggy
• Neyret, Aymeric
• Popp, Jurgen
• Horvat, Branka
• Muriaux, Delphine
• Cuisinier, Frederic

Titel

Specific intracellular signature of SARS-CoV-2 infection using confocal Raman microscopy

Ist Teil von

• Communications chemistry, 2022-07, Vol.5 (1), p.85

Ort / Verlag

England

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• SARS-CoV-2 infection remains spread worldwide and requires a better understanding of virus-host interactions. Here, we analyzed biochemical modifications due to SARS-CoV-2 infection in cells by confocal Raman microscopy. Obtained results were compared with the infection with another RNA virus, the measles virus. Our results have demonstrated a virus-specific Raman molecular signature, reflecting intracellular modification during each infection. Advanced data analysis has been used to distinguish non-infected versus infected cells for two RNA viruses. Further, classification between non-infected and SARS-CoV-2 and measles virus-infected cells yielded an accuracy of 98.9 and 97.2 respectively, with a significant increase of the essential amino-acid tryptophan in SARS-CoV-2-infected cells. These results present proof of concept for the application of Raman spectroscopy to study virus-host interaction and to identify factors that contribute to the efficient SARS-CoV-2 infection and may thus provide novel insights on viral pathogenesis, targets of therapeutic intervention and development of new COVID-19 biomarkers.