Details

Autor(en) / Beteiligte

• Sahu, Soumyadip
• Gordon, Jacob
• Gu, Chunfang
• Sobhany, Mack
• Fiedler, Dorothea
• Stanley, Robin E
• Shears, Stephen B

Titel

Nucleolar Architecture Is Modulated by a Small Molecule, the Inositol Pyrophosphate 5-InsP 7

Ist Teil von

• Biomolecules (Basel, Switzerland), 2023-01, Vol.13 (1)

Ort / Verlag

Switzerland

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Inositol pyrophosphates (PP-InsPs); are a functionally diverse family of eukaryotic molecules that deploy a highly-specialized array of phosphate groups as a combinatorial cell-signaling code. One reductive strategy to derive a molecular-level understanding of the many actions of PP-InsPs is to individually characterize the proteins that bind them. Here, we describe an alternate approach that seeks a single, collective rationalization for PP-InsP binding to an entire group of proteins, i.e., the multiple nucleolar proteins previously reported to bind 5-InsP (5-diphospho-inositol-1,2,3,4,6-pentakisphosphate). Quantitative confocal imaging of the outer nucleolar granular region revealed its expansion when cellular 5-InsP levels were elevated by either (a) reducing the 5-InsP metabolism by a CRISPR-based knockout (KO) of either or s; or (b), the heterologous expression of wild-type inositol hexakisphosphate kinase, i.e., IP6K2; separate expression of a kinase-dead IP6K2 mutant did not affect granular volume. Conversely, the nucleolar granular region in KO cells shrank back to the wild-type volume upon attenuating 5-InsP synthesis using either a pan-IP6K inhibitor or the siRNA-induced knockdown of IP6K1+IP6K2. Significantly, the inner fibrillar volume of the nucleolus was unaffected by 5-InsP . We posit that 5-InsP acts as an 'electrostatic glue' that binds together positively charged surfaces on separate proteins, overcoming mutual protein-protein electrostatic repulsion the latter phenomenon is a known requirement for the assembly of a non-membranous biomolecular condensate.