Details

Autor(en) / Beteiligte

• Hussain, E A
• Qasim Hameed, H
• Mujahid Al-Shuwaikh, A
• Mujahid Abdullah, R

Titel

Detection of the aadA1 and aac (3)-1V resistance genes in Acinetobacter baumannii

Ist Teil von

• Archives of Razi Institute, 2022-06, Vol.77 (3), p.959

Ort / Verlag

Iran

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• is a gram-negative aerobic bacterium that can be found in different environments, such as food, containing vegetables, meat, and fish; moreover, it can be present in soil and freshwater. has globally considered an opportunistic nosocomial bacterium in the healthcare setting contributing to increased morbidity and mortality. The current study aimed to detect the aminoglycoside genes in isolated from different clinical causes. In total, 20 isolates of were obtained from different clinical cases. Bacterial isolate DNA was extracted using a DNA extraction kit. Quantus Fluorometer was used to detect the concentration of the extracted DNA in order to detect the goodness of samples. 1 μl of DNA and 199 μl of diluted QuantiFlour Dye were mixed. After 5 min incubation at room temperature, DNA concentration values were evaluated, and following the initial amplification of the gene, 20 μl of PCR product with F and R primers were sent to Sanger sequencing. The results of the antimicrobial susceptibility revealed that isolates were resistant to Gentamicin (95%), Amikacin (90%), and Tobramycin (60%). Molecular investigation of the and genes exhibited that the gene was detected in 15% of the isolates. However, the gene was not detected in any of the isolates. The gel electrophoresis revealed that the molecular weight of the gene was 490bp. The DNA sequence of the gene was conducted in this study, and the results exhibited no mutations in all isolates.