Details

Autor(en) / Beteiligte

• Luo, Yi
• Kong, Hui-Min
• Su, Wei-Qing
• Yi, Wen-Fang
• Ge, Hui
• Chen, Hui
• Li, Liang
• Yang, Mo

Titel

The Hematopoietic Protective Effect of PDGF-BB on Radiation-Induced Myelosuppression Model Mice

Ist Teil von

• Zhongguo shi yan xue ye xue za zhi, 2022-12, Vol.30 (6), p.1873

Ort / Verlag

China

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the hematopoietic protective effect of platelet-derived growth factor (PDGF)-BB on radiation-induced myelosuppression model mice and effect of anti-apoptosis of megakaryocyte line Meg-01 cells, and its possible mechanism. Mice were radiated with 4 Gy of Csγ ray to establish the model of myelosuppression. Mice were weighed and peripheral blood cell were counted before radiation (day 0) and day 7, 14 and 21 after radiation. On the 21 day, the mice were killed. The sternal tissues of the mice were taken for morphological observation, and the femoral bone marrow cells were cultured for the assay of colony cell forming units (CFU). Meg-01 cells were cultured without FBS for 24 h to induce apoptosis, and then treated with PDGF-BB for 48 h. The effects of PDGF-BB on the proliferation were investigated by cell counting. Flow cytometry was used to detect early apoptosis (Annexin V), mitochondrial membrane potential (JC-1) and the expression of caspase-3. Peripheral blood cell counts of mice showed that