Details

Autor(en) / Beteiligte

• Varinelli, Luca
• Guaglio, Marcello
• Brich, Silvia
• Zanutto, Susanna
• Belfiore, Antonino
• Zanardi, Federica
• Iannelli, Fabio
• Oldani, Amanda
• Costa, Elisa
• Chighizola, Matteo
• Lorenc, Ewelina
• Minardi, Simone P
• Fortuzzi, Stefano
• Filugelli, Martina
• Garzone, Giovanna
• Pisati, Federica
• Vecchi, Manuela
• Pruneri, Giancarlo
• Kusamura, Shigeki
• Baratti, Dario
• Cattaneo, Laura
• Parazzoli, Dario
• Podestà, Alessandro
• Milione, Massimo
• Deraco, Marcello
• Pierotti, Marco A
• Gariboldi, Manuela

Titel

Decellularized extracellular matrix as scaffold for cancer organoid cultures of colorectal peritoneal metastases

Ist Teil von

• Journal of molecular cell biology, 2023-04, Vol.14 (11)

Ort / Verlag

United States

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Peritoneal metastases (PM) from colorectal cancer (CRC) are associated with poor survival. The extracellular matrix (ECM) plays a fundamental role in modulating the homing of CRC metastases to the peritoneum. The mechanisms underlying the interactions between metastatic cells and the ECM, however, remain poorly understood, and the number of in vitro models available for the study of the peritoneal metastatic process is limited. Here, we show that decellularized ECM of the peritoneal cavity allows the growth of organoids obtained from PM, favoring the development of three-dimensional (3D) nodules that maintain the characteristics of in vivo PM. Organoids preferentially grow on scaffolds obtained from neoplastic peritoneum, which are characterized by greater stiffness than normal scaffolds. A gene expression analysis of organoids grown on different substrates reflected faithfully the clinical and biological characteristics of the organoids. An impact of the ECM on the response to standard chemotherapy treatment for PM was also observed. The ex vivo 3D model, obtained by combining patient-derived decellularized ECM with organoids to mimic the metastatic niche, could be an innovative tool to develop new therapeutic strategies in a biologically relevant context to personalize treatments.