Details

Autor(en) / Beteiligte

• Dhillon-Richardson, Rekha M
• Haugan, Alexandra K
• Martik, Megan L

Titel

Fishing for Developmental Regulatory Regions: Zebrafish Tissue-Specific ATAC-seq

Ist Teil von

• Methods in molecular biology (Clifton, N.J.), 2023, Vol.2599, p.271

Ort / Verlag

United States

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Interactions between transcription factors and regulatory DNA can be described by gene regulatory networks. These networks provide a systems-level view of embryonic tissue development. Here, we describe a protocol for the isolation, identification, and experimental manipulation of tissue-specific cis-regulatory elements during zebrafish embryonic development using low-input ATAC-seq. With the methods described, genome-wide assessments of regulatory DNA in small populations of developing tissues can be identified, allowing for the construction of gene regulatory networks.