Details

Autor(en) / Beteiligte

• Huang, Xiaofang
• Xu, Liangting
• Bi, Chuyun
• Zhao, Lili
• Zhang, Limei
• Chen, Xuanyang
• Qi, Shiqian
• Lin, Shiqiang

Titel

Designing Overlap Extension PCR Primers for Protein Mutagenesis: A Programmatic Approach

Ist Teil von

• Methods in molecular biology (Clifton, N.J.), 2022, Vol.2461, p.1

Ort / Verlag

United States

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• Overlap extension PCR is one of the routinely used methods to generate mutagenic genes for the functional and structural study of proteins. However, it is time-consuming to design the overlapping mutagenic primers and gene primers by manual operation. In this chapter, we present a Python script that is able to search all the possible primer combinations according to the preset definitions and calculate the necessary parameters of each primer for the users, which could facilitate the primer design process. Up to 256 pairs of primers can be provided for selection using this script.