Details

Autor(en) / Beteiligte

• Han, Guangchun
• Deng, Qing
• Marques-Piubelli, Mario L
• Dai, Enyu
• Dang, Minghao
• Ma, Man Chun John
• Li, Xubin
• Yang, Haopeng
• Henderson, Jared
• Kudryashova, Olga
• Meerson, Mark
• Isaev, Sergey
• Kotlov, Nikita
• Nomie, Krystle J
• Bagaev, Alexander
• Parra, Edwin R
• Solis Soto, Luisa M
• Parmar, Simrit
• Hagemeister, Fredrick B
• Ahmed, Sairah
• Iyer, Swaminathan P
• Samaniego, Felipe
• Steiner, Raphael
• Fayad, Luis
• Lee, Hun
• Fowler, Nathan H
• Flowers, Christopher R
• Strati, Paolo
• Westin, Jason R
• Neelapu, Sattva S
• Nastoupil, Loretta J
• Vega, Francisco
• Wang, Linghua
• Green, Michael R

Titel

Follicular Lymphoma Microenvironment Characteristics Associated with Tumor Cell Mutations and MHC Class II Expression

Ist Teil von

• Blood cancer discovery, 2022-09, Vol.3 (5), p.428-443

Ort / Verlag

United States

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• Follicular lymphoma (FL) is a B-cell malignancy with a complex tumor microenvironment that is rich in nonmalignant immune cells. We applied single-cell RNA sequencing to characterize the diverse tumor and immune cell populations of FL and identified major phenotypic subsets of FL T cells, including a cytotoxic CD4 T-cell population. We characterized four major FL subtypes with differential representation or relative depletion of distinct T-cell subsets. By integrating exome sequencing, we observed that somatic mutations are associated with, but not definitive for, reduced MHC expression on FL cells. In turn, expression of MHCII genes by FL cells was associated with significant differences in the proportions and targetable immunophenotypic characteristics of T cells. This provides a classification framework of the FL microenvironment in association with FL genotypes and MHC expression, and informs different potential immunotherapeutic strategies based upon tumor cell MHCII expression. We have characterized the FL-infiltrating T cells, identified cytotoxic CD4 T cells as an important component that is associated with tumor cell-intrinsic characteristics, and identified sets of targetable immune checkpoints on T cells that differed from FLs with normal versus low MHC expression. See related commentary by Melnick, p. 374. This article is highlighted in the In This Issue feature, p. 369.