Zhōnghuá yùfáng-yīxué zázhì, 2022-05, Vol.56 (5), p.601
2022
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Details
- Autor(en) / Beteiligte
- Titel
- Transcriptomic analysis of the ΔPaLoc mutant of Clostridioides difficile and verification of its toxicity
- Ist Teil von
- Zhōnghuá yùfáng-yīxué zázhì, 2022-05, Vol.56 (5), p.601
- Ort / Verlag
- China
- Erscheinungsjahr
- 2022
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Comparative analyses of wild-type 630 (Cd630) strain and pathogenicity locus (PaLoc) knockout mutant (ΔPaLoc) by using RNA-seq technology. Analysis of differential expression of Cd630 wild-type strain and ΔPaLoc mutant strain and measurement of its cellular virulence changes. Lay the foundation for the construction of an toxin-attenuated vaccine strain against Analysis of Cd630 and ΔPaLoc mutant strains using high-throughput sequencing (RNA-seq). Clustering differentially expressed genes and screening differentially expressed genes by DESeq software. Further analysis of differential genes using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. Finally, cytotoxicity assays of ΔPaLoc and Cd630 strains were performed in the African monkey kidney epithelial cell (Vero) and the human colonic cell (Caco-2) lines. The transcriptome data showed that the ΔPaLoc mutant toxin genes and were not transcribed. Compared to the wild-type strain, and genes upregulated 17.92,11.40,8.93 and 7.55
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 0253-9624DOI: 10.3760/cma.j.cn112150-20220222-00166Titel-ID: cdi_pubmed_primary_35644974
- Format
- –
- Schlagworte
- Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Bacterial Toxins - genetics, Bacterial Toxins - metabolism, Caco-2 Cells, Clostridioides, Clostridioides difficile - genetics, Humans, Oxidoreductases - genetics, Oxidoreductases - metabolism, Transcriptome, Vaccines, Attenuated