Details

Autor(en) / Beteiligte

• Meng, C Y
• Gong, X L
• Zhao, R
• Lu, Q
• Dong, X Y

Titel

Effect of maternal exposure to lipopolysaccharide during pregnancy on allergic asthma in offspring in mice

Ist Teil von

• Zhonghua er ke za zhi, 2022-04, Vol.60 (4), p.302

Ort / Verlag

China

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the effect of maternal exposure to lipopolysaccharide during pregnancy on allergic asthma in offspring in mice. Animal experimental research was carried out from June 2019 to June 2021.Pregnant C57BL/6J mice were randomly divided into 2 groups by intraperitoneal injection with 7 μg/kg lipopolysaccharide (LPS) or phosphate buffered saline (PBS) at day 15.5 of gestation. After birth, 6 offspring were randomly chosen from each group at the age of 4 weeks, and stimulated with house dust mites (HDM) or PBS, further divided into 4 groups, such as LPS+PBS group, LPS+HDM group, PBS+PBS group, PBS+HDM group, with 3 mice in each group. The cough and wheezing were observed, the histological changes in lung tissue were examined after HE staining, and the expression of inflammatory factors including interleukin (IL)-4, IL-6, IL-17A, IL-23, interferon (IFN)-α and IFN-β in the lung tissue were detected by high-throughput liquid protein chip detection. test or rank sum test was used for the comparison among th