Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Effect of maternal exposure to lipopolysaccharide during pregnancy on allergic asthma in offspring in mice
Ist Teil von
Zhonghua er ke za zhi, 2022-04, Vol.60 (4), p.302
Ort / Verlag
China
Erscheinungsjahr
2022
Quelle
MEDLINE
Beschreibungen/Notizen
To investigate the effect of maternal exposure to lipopolysaccharide during pregnancy on allergic asthma in offspring in mice.
Animal experimental research was carried out from June 2019 to June 2021.Pregnant C57BL/6J mice were randomly divided into 2 groups by intraperitoneal injection with 7 μg/kg lipopolysaccharide (LPS) or phosphate buffered saline (PBS) at day 15.5 of gestation. After birth, 6 offspring were randomly chosen from each group at the age of 4 weeks, and stimulated with house dust mites (HDM) or PBS, further divided into 4 groups, such as LPS+PBS group, LPS+HDM group, PBS+PBS group, PBS+HDM group, with 3 mice in each group. The cough and wheezing were observed, the histological changes in lung tissue were examined after HE staining, and the expression of inflammatory factors including interleukin (IL)-4, IL-6, IL-17A, IL-23, interferon (IFN)-α and IFN-β in the lung tissue were detected by high-throughput liquid protein chip detection.
test or rank sum test was used for the comparison among th