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A faecal microbiota signature with high specificity for pancreatic cancer
Gut, 2022-07, Vol.71 (7), p.1359-1372
2022
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Autor(en) / Beteiligte
Titel
A faecal microbiota signature with high specificity for pancreatic cancer
Ist Teil von
  • Gut, 2022-07, Vol.71 (7), p.1359-1372
Ort / Verlag
England: BMJ Publishing Group Ltd and British Society of Gastroenterology
Erscheinungsjahr
2022
Quelle
MEDLINE
Beschreibungen/Notizen
  • BackgroundRecent evidence suggests a role for the microbiome in pancreatic ductal adenocarcinoma (PDAC) aetiology and progression.ObjectiveTo explore the faecal and salivary microbiota as potential diagnostic biomarkers.MethodsWe applied shotgun metagenomic and 16S rRNA amplicon sequencing to samples from a Spanish case–control study (n=136), including 57 cases, 50 controls, and 29 patients with chronic pancreatitis in the discovery phase, and from a German case–control study (n=76), in the validation phase.ResultsFaecal metagenomic classifiers performed much better than saliva-based classifiers and identified patients with PDAC with an accuracy of up to 0.84 area under the receiver operating characteristic curve (AUROC) based on a set of 27 microbial species, with consistent accuracy across early and late disease stages. Performance further improved to up to 0.94 AUROC when we combined our microbiome-based predictions with serum levels of carbohydrate antigen (CA) 19–9, the only current non-invasive, Food and Drug Administration approved, low specificity PDAC diagnostic biomarker. Furthermore, a microbiota-based classification model confined to PDAC-enriched species was highly disease-specific when validated against 25 publicly available metagenomic study populations for various health conditions (n=5792). Both microbiome-based models had a high prediction accuracy on a German validation population (n=76). Several faecal PDAC marker species were detectable in pancreatic tumour and non-tumour tissue using 16S rRNA sequencing and fluorescence in situ hybridisation.ConclusionTaken together, our results indicate that non-invasive, robust and specific faecal microbiota-based screening for the early detection of PDAC is feasible.
Sprache
Englisch
Identifikatoren
ISSN: 0017-5749
eISSN: 1468-3288
DOI: 10.1136/gutjnl-2021-324755
Titel-ID: cdi_pubmed_primary_35260444
Format
–
Schlagworte
Accuracy, Adenocarcinoma, Biomarkers, Biomarkers, Tumor, CA-19-9 Antigen, cancer prevention, Carcinoma, Pancreatic Ductal - diagnosis, Carcinoma, Pancreatic Ductal - genetics, Case-Control Studies, FDA approval, Humans, Hybridization, intestinal microbiology, Metagenomics, Microbiomes, Microbiota, Pancreas, Pancreatic cancer, Pancreatic Neoplasms, Pancreatic Neoplasms - diagnosis, Pancreatic Neoplasms - genetics, pancreatic tumours, Pancreatitis, Population studies, RNA, Ribosomal, 16S - genetics, rRNA 16S, Saliva, screening, Serum levels, Species, Tumors

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