Details

Autor(en) / Beteiligte

• Dagälv, Anders
• Lundequist, Anders
• Filipek-Górniok, Beata
• Dierker, Tabea
• Eriksson, Inger
• Kjellén, Lena

Titel

Heparan Sulfate Structure: Methods to Study N-Sulfation and NDST Action

Ist Teil von

• Methods in molecular biology (Clifton, N.J.), 2022, Vol.2303, p.139

Ort / Verlag

United States

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• Heparan sulfate proteoglycans are important modulators of cellular processes where the negatively charged polysaccharide chains interact with target proteins. The sulfation pattern of the heparan sulfate chains will determine which proteins will bind and the affinity of the interactions. The N-deacetylase/N-sulfotransferase (NDST) enzymes are of key importance during heparan sulfate biosynthesis when the sulfation pattern is determined. In this chapter, metabolic labeling of heparan sulfate with [ S]sulfate or [ H]glucosamine in cell cultures is described, in addition to characterization of polysaccharide chain length and degree of N-sulfation. Methods to measure NDST enzyme activity are also presented.