Details

Autor(en) / Beteiligte

• Marguerite M. B. Kay
• Gieljan J. C. G. M. Bosman
• Shapiro, Stanley S.
• Bendich, Adrianne
• Bassel, Paul S.

Titel

Oxidation as a Possible Mechanism of Cellular Aging: Vitamin E Deficiency Causes Premature Aging and IgG Binding to Erythrocytes

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 1986-04, Vol.83 (8), p.2463-2467

Ort / Verlag

Washington, DC: National Academy of Sciences of the United States of America

Erscheinungsjahr

1986

Link zum Volltext

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• Senescent-cell antigen is a ``neo-antigen'' that appears on the surface of senescent cells and initiates IgG binding and cellular removal. As an approach to evaluating oxidation as a possible mechanism for generation of senescentcell antigen, we studied erythrocytes from vitamin E-deficient rats. Vitamin E is localized primarily in cellular membranes. Its major role is the termination of free-radical chain reactions propagated by the polyunsaturated fatty acids of membrane phospholipids. Results of our studies indicate that erythrocytes of all ages from vitamin E-deficient rats behave like old erythrocytes from normal rats, as determined by their susceptibility to phagocytosis, IgG binding, anion transport ability, and glyceraldehyde-3-phosphate dehydrogenase activity. Increased breakdown products of band 3 were observed with immunoblotting in membranes of erythrocytes from vitamin E-deficient rats. Breakdown products of band 3 are known to increase as cells age in normal individuals. The data suggest that oxidation may be a possible mechanism for erythrocyte aging and generation of senescent-cell antigen in vivo.