Details

Autor(en) / Beteiligte

• Reyes Serratos, Eduardo Alejandro
• Fernandez Castillo, Elisa
• Sanchez Lopez, Lizeth
• Salinas Santander, Mauricio
• Orozco Nunnelly, Danielle Annette
• Sanchez Salazar, Monica Gabriela
• Cervantes Astorga, Enrique
• Rivas Estilla, Ana Maria
• Rios Ibarra, Clara Patricia

Titel

Down regulation of tumour biomarkers in colon cancer cells with IRNA PFK-1 plus metformin

Ist Teil von

• Journal of B.U.ON. : official journal of the Balkan Union of Oncology, 2021-07, Vol.26 (4), p.1210

Ort / Verlag

Cyprus

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Metformin has been widely used for the treatment of Type 2 Diabetes Mellitus (T2DM), hyperglycemia and polycystic ovarian syndrome. Recent studies have suggested the potential of this substance as a cancer chemopreventive agent. We evaluated the antitumoral effect of iRNA-PFK-1 and the combined therapy iRNA-PFK-1 + metformin in RKO p53-positive cells. mRNA levels of tumor suppressor genes AMPK, APC, and c-MYC, KRAS oncogenes were measured by qRT-PCR in RKO cells treated with 25 µM metformin alone or combined with iRNA-PFK-1, to evaluate the effect of both treatments. At 72 h after treatment with either 25 µM metformin, 150 nM iRNA-PFK-1, or the combined treatment, the transcriptional levels of these biomarkers were decreased by ~73% (p˂0.05), ~99.9%, (p˂0.01), and ~76% (p˂0.05), respectively. These in vitro results support the potential therapeutic role of metformin and PFK-1 in the treatment of colon cancer via down-modulation of the expression of several important cancer biomarkers.