Details

Autor(en) / Beteiligte

• Junius, Steffie
• Mavrogiannis, Adamantios V
• Lemaitre, Pierre
• Gerbaux, Margaux
• Staels, Frederik
• Malviya, Vanshika
• Burton, Oliver
• Gergelits, Václav
• Singh, Kailash
• Tito Tadeo, Raul Yhossef
• Raes, Jeroen
• Humblet-Baron, Stephanie
• Liston, Adrian
• Schlenner, Susan M

Titel

Unstable regulatory T cells, enriched for naïve and Nrp1 neg cells, are purged after fate challenge

Ist Teil von

• Science immunology, 2021-07, Vol.6 (61)

Ort / Verlag

United States

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Regulatory T cells (T ) are indispensable for the control of immune homeostasis and have clinical potential as a cell therapy for treating autoimmunity. T can lose expression of the lineage-defining Foxp3 transcription factor and acquire effector T cell (T ) characteristics, a process referred to as T plasticity. The extent and reversibility of such plasticity during immune responses remain unknown. Here, using a murine genetic fate-mapping system, we show that T stability is maintained even during exposure to a complex microbial/antigenic environment. Furthermore, we demonstrate that the observed plasticity of T after adoptive transfer into a lymphopenic environment is a property limited to only a subset of the T population, with the nonconverting majority of T being resistant to plasticity upon secondary stability challenge. The unstable T fraction is a complex mixture of phenotypically distinct T , enriched for naïve and neuropilin-1-negative T , and includes peripherally induced T and recent thymic emigrant T These results suggest that a "purging" process can be used to purify stable T that are capable of robust fate retention, with potential implications for improving cell transfer therapy.