Details

Autor(en) / Beteiligte

• Nayfach, Stephen
• Páez-Espino, David
• Call, Lee
• Low, Soo Jen
• Sberro, Hila
• Ivanova, Natalia N
• Proal, Amy D
• Fischbach, Michael A
• Bhatt, Ami S
• Hugenholtz, Philip
• Kyrpides, Nikos C

Titel

Metagenomic compendium of 189,680 DNA viruses from the human gut microbiome

Ist Teil von

• Nature microbiology, 2021-07, Vol.6 (7), p.960-970

Ort / Verlag

England

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Bacteriophages have important roles in the ecology of the human gut microbiome but are under-represented in reference databases. To address this problem, we assembled the Metagenomic Gut Virus catalogue that comprises 189,680 viral genomes from 11,810 publicly available human stool metagenomes. Over 75% of genomes represent double-stranded DNA phages that infect members of the Bacteroidia and Clostridia classes. Based on sequence clustering we identified 54,118 candidate viral species, 92% of which were not found in existing databases. The Metagenomic Gut Virus catalogue improves detection of viruses in stool metagenomes and accounts for nearly 40% of CRISPR spacers found in human gut Bacteria and Archaea. We also produced a catalogue of 459,375 viral protein clusters to explore the functional potential of the gut virome. This revealed tens of thousands of diversity-generating retroelements, which use error-prone reverse transcription to mutate target genes and may be involved in the molecular arms race between phages and their bacterial hosts.