Details

Autor(en) / Beteiligte

• Fan, Pengju
• Fang, Man
• Li, Jingjing
• Solari, Mario G
• Wu, Dingyu
• Tan, Wuyuan
• Wang, Yongjie
• Yang, Xinghua
• Lei, Shaorong

Titel

A Novel Fat Making Strategy for Adipose-Derived Progenitor Cells Enrichment Fat Improves Fat Graft Survival

Ist Teil von

• Aesthetic surgery journal, 2021-05

Ort / Verlag

England

Erscheinungsjahr

2021

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• A low survival rate is one of the main challenges in fat grafting. This study aimed to evaluate whether the microfat obtained using a novel strategy promoted the survival and retention of fat grafts. A 5 mm diameter blunt tip cannula with larger side holes (~30 mm 2 per hole) was used to obtain macrofat. Subsequently, a novel strategy based on a newly invented extracorporeal cutting device was used to cut the macrofat into microfat, which was named adipose-derived progenitor cells enrichment fat (AER fat); Coleman fat was used as the control. Aliquots (0.5 mL) of both groups of fat were transplanted into 10 nude mice and analyzed 10 weeks later. Western blotting, flow cytometry, and immunofluorescence were performed to assess the AER characteristics and underlying mechanisms. The retention rate of fat grafts in AER fat-treated animals was significantly higher than that in the Coleman group (54.6±13% vs. 34.8±9%, P<0.05) after 10 weeks. AER fat contained more DPP4 + progenitor cells (3.3±0.61×10 3 vs. 2.0±0.46×10 3 cells/mL, P<0.05), adipose-derived plastic-adherent cells (6.0±1.10×10 4 vs. 2.6±0.17×10 4 cells/mL, P<0.001), and viable adipocytes as compared to Coleman fat. Moreover, histological analysis showed that AER fat graft had better histological structure and higher capillary density. AER fat transplantation is a potential strategy to improve the survival and long-term retention of fat grafts. AER fat contained more DPP4 + progenitor cells.