Details

Autor(en) / Beteiligte

• Wang, Changjian
• Zhang, Lei
• Luo, Jiaoyang
• Qin, Jiaan
• Jiang, Jiayi
• Qin, Lu
• Zhao, Zhigao
• Yang, Shihai
• Yang, Meihua

Titel

Development of a sensitive indirect competitive enzyme-linked immunosorbent assay for high-throughput detection and risk assessment of aflatoxin B 1 in animal-derived medicines

Ist Teil von

• Toxicon (Oxford), 2021-07, Vol.197, p.99

Ort / Verlag

England

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Animal-derived medicine is an important part of traditional Chinese medicine (TCM). Studies have shown that many animal-derived medicinal products are susceptible to contaminate of aflatoxins, nevertheless, the rapid detection for animal-derived medicine is prone to be ignored. Here we developed a sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) for rapid screening of aflatoxin B (AFB ) in ground beetle, cockroach, silkworm and earthworm. The sensitivity of the icELISA method was significantly enhanced. The IC for the four animal-derived medicinal samples ranged from 0.092 to 0.135 ng mL ; the limit of detection (LOD) was 0.008-0.020 ng mL . To obtain high accuracy, the extraction solution and time were evaluated. By using this method, a total of 138 samples were investigated, and the detection rates of AFB in ground beetle and earthworm samples were 26.6% and 16.7%, respectively. The result was validated by liquid chromatography combined with tandem mass spectrometry, and an excellent correlation was observed between the two datasets, with a R value of 0.999. Our results indicate that the proposed method can be used for the rapid detection of AFB in animal-derived medicine. Furthermore, the quantitative risk assessment was conducted for ground beetle and earthworm based on the results, demonstrating that the intake of AFB in ground beetle had a slight threat to the risk of cancer.