Details

Autor(en) / Beteiligte

• Bai, Chun-Hong
• Zhang, Wen-Li
• Hu, Shi-Zhong

Titel

Sacral nerve stimulating on intestinal mucosal immune barrier function of rats with acute complete spinal cord injury

Ist Teil von

• Zhongguo ying yong sheng li xue za zhi, 2020-11, Vol.36 (6), p.539

Ort / Verlag

China

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• To study the effects of sacral nerve root stimulation on intestinal mucosal immune barrier function in rat with acute complete spinal cord injury(SCI). Fifty-six Wistar rats were divided into Sham group(SG =8), control group(CG 24 、 48、 72 h, =8), and experimental group(EG 24、 48、 72 h, =8). In CG and EG, according to Fehlings'method,we transected the spinal cord by the aneurysm clip and implanted electrodes into the third sacral foramina on the right side.We stimulated in intensity 4 V,the frequency of 15 Hz,and the pulse of 210 μs.The stimulation period was 2 hours,with 10 minutes stimulation and 10 minutes rest intermittently,twice a day at 8:00-10:00 am and 6:00-8:00 pm. The intestinal morphology was observed under light microscope and electron microscope. The protein expression levels of A20,NOD2,and CD68 by Western blot . ① SCI caused impaired intestinal epithelial barrier function. The intestinal mucosa appeared different degree of damage in CG group; cell-cell connections between intestinal epithelial