Methods in molecular biology (Clifton, N.J.), 2021, Vol.2270, p.437
2021
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Details
- Autor(en) / Beteiligte
- Titel
- Analysis of Regulatory B Cells in Experimental Autoimmune Uveitis
- Ist Teil von
- Methods in molecular biology (Clifton, N.J.), 2021, Vol.2270, p.437
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2021
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Regulatory B cells (Bregs) that produce IL-35 and IL-10 (i35-Bregs) regulate central nervous system (CNS) autoimmune diseases including uveitis. In the mouse model of uveitis, i35-Breg cells suppress intraocular inflammation by inducing expansion of IL-10-producing B cells (B10), IL-10-producing T cells (Tregs), and IL-35-producing T cells (iT 35), suggesting that i35-Bregs orchestrate an immune-suppressive milieu that regulates immunity during autoimmune diseases. In this chapter, we discuss uveitis and therapeutic challenges that necessitate the development of cell-based therapies for the treatment of these potentially blinding diseases that cause 10% visual handicap. We then describe the methods we set up for ex vivo generation of i35-Breg cells employed in i35-Breg immunotherapy in uveitis and in other CNS autoimmune diseases.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1940-6029DOI: 10.1007/978-1-0716-1237-8_23Titel-ID: cdi_pubmed_primary_33479912
- Format
- –
- Schlagworte
- Adoptive Transfer, Animals, Autoimmune Diseases - immunology, B-Lymphocytes - cytology, B-Lymphocytes - immunology, B-Lymphocytes, Regulatory - cytology, B-Lymphocytes, Regulatory - immunology, B-Lymphocytes, Regulatory - pathology, Central Nervous System Diseases, Disease Models, Animal, Female, Inflammation - pathology, Interleukin-10 - immunology, Male, Mice, Mice, Inbred NOD, T-Lymphocytes, Regulatory - immunology, Uveitis - immunology, Uveitis - metabolism, Uveitis - therapy