Details

Autor(en) / Beteiligte

• Li, Zhao
• Chen, Yizhi
• Meng, Wei
• Yang, Zhiran
• Lin, Yulin
• Zhang, Jue
• Sun, Pingping
• DU, Xuemei
• Qu, Congling
• Chang, Hong
• Wei, Jixue
• Li, Yan

Titel

Consistency of two commercial secondary antibodies for immunohistochemistry

Ist Teil von

• Xi bao yu fen zi mian yi xue za zhi, 2021-01, Vol.37 (1), p.47

Ort / Verlag

China

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Objective To compare the consistency of immunohistochemical staining between the two commercial secondary antibodies. Methods Eighteen common immunohistochemical primary antibodies were selected and positive and negative controls were set up according to the recommendations from the AD Hoc Committee of International Experts. Under the same experimental conditions, the DAKO automatic immunohistochemical staining platform was used to test two different secondary antibodies for immunohistochemical staining. The standard group for the secondary antibody was provided by the DAKO polymer system (DAKO EnVision FLEX, High pH), and the experimental group for the secondary antibody was provided by the Power-Stain kit (Power-Stain 1.0 Poly HRP DAB Kit for Mouse+Rabbit). Subsequently, the images were captured. A single-blind, positioning, qualitative and semi-quantitative scoring criterion was used for describing the positive stains by the experienced pathologist. Absorbance corrected values, measured area values and pos