Methods in molecular biology (Clifton, N.J.), 2021, Vol.2261, p.421
2021
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Details
- Autor(en) / Beteiligte
- Titel
- Investigating the Adipose Tissue Secretome: A Protocol to Generate High-Quality Samples Appropriate for Comprehensive Proteomic Profiling
- Ist Teil von
- Methods in molecular biology (Clifton, N.J.), 2021, Vol.2261, p.421
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2021
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- In this chapter we describe in detail how to prepare a sample containing the complete set of secretion products from primary adipocytes, which are suitable for comprehensive and sensitive secretome analysis. The underlying protocol should be seen as starting point guiding through critical steps of the complex workflow of preperation of secretomes. For diverse research questions and in the context of different sample types used, the protocol has to be carefully adjusted in order to approximate to the real secretome.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1940-6029DOI: 10.1007/978-1-0716-1186-9_26Titel-ID: cdi_pubmed_primary_33421005
- Format
- –
- Schlagworte
- Adipocytes - metabolism, Adipose Tissue - cytology, Adipose Tissue - metabolism, Analytic Sample Preparation Methods, Animals, Cell Separation, Cells, Cultured, Electrophoresis, Gel, Two-Dimensional, Humans, Primary Cell Culture, Proteins - analysis, Proteome, Proteomics, Secretory Pathway, Tandem Mass Spectrometry, Workflow