Details

Autor(en) / Beteiligte

• Blundell, Michael P
• Sanderson, Sharon L
• Long, Tracey A

Titel

Flow Cytometry as an Important Tool in Proteomic Profiling

Ist Teil von

• Methods in molecular biology (Clifton, N.J.), 2021, Vol.2261, p.213

Ort / Verlag

United States

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Flow cytometry enables the simultaneous detection of multiple surface and intracellular antigens for proteomic profiling of cells. This allows characterization and identification of specific cell subtypes within a heterogeneous population and is usually called immunophenotyping. Antigen-specific antibodies, conjugated to various fluorophores, are incubated with the sample to identify each marker. Fluorescent light of various wavelengths can be separated, detected, and converted into a digital signal in a flow cytometer. Here we describe an eight-color experiment to identify key peripheral blood cell types; however, this technique can be expanded to detect more than 30 parameters simultaneously.